S5a protein

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August undefined, 2024

Web26S proteasome non-ATPase regulatory subunit 4, Antisecretory factor 1, Multiubiquitin chain-binding protein : Clone: S5a-18 : Host: Mouse : Isotype: IgG1 : Immunogen: Recombinant full-length human Rpn10 protein. UniProt ID: P55036 : Gene/Protein Identifier: PSMD4 (gene name) Source: Purified from hybridoma tissue culture supernatant. Species ... WebNational Center for Biotechnology Information

1P1A: NMR structure of ubiquitin-like domain of hHR23B - RCSB

WebS5a is one of the integral Ub receptors of the 26S proteasome. It’s N-terminal vWA domain interacts with Rpn9 and Rpn10 on the 26S proteasome. The C-terminal domain of S5a … WebOct 14, 2013 · S5a is required for the degradation of p53 but not its E3 ligase Mdm2. Additional work is required to completely define the pathways of proteasomal recognition … the union eagan

Novel Regulation of the Helix-Loop-Helix Protein Id1 by S5a, a …

WebSep 7, 2009 · Particular attention was paid to the 19S-RP protein, S5a/PSMD4, and the 20S-CP protein, α-5/PSMA5, expected to be involved in the exaggerated proteasome activity in cancer (Murata et al., 2009). WebThe sequence depicted encodes the entire S5a protein beginning with potential initiation Met codons at positions 27 and 54, respectively. The original pACT-derived cDNA, clone 14A, that was isolated based on the interaction of its encoded protein with Id1 begins at codon 34. The entire reading frame encodes a protein of 377 amino acids. WebJun 15, 2015 · The HIS-tagged human S5a protein was expressed in bacteria following cloning of the entire open reading frame (377 aa) in the pET-21a (Novagen, Billerica, MA) vector. The plasmids were transformed into E. coli BL21 (DE3) and protein expression was induced with 1.0 mM IPTG for 4 hrs at 37°C. the union eatery okc

Increased proteasome subunit protein expression and proteasome activity …

DNA-repair protein hHR23a alters its protein structure upon …

WebOct 13, 2003 · In this work, we illustrate how the proteasomal subunit S5a regulates hHR23a protein structure. By using NMR spectroscopy, we have elucidated the structure and … WebOct 13, 2003 · The Binding Surfaces of S5a and the UBA Domains Overlap. We used an S5a construct spanning residues M196 to D307 to study the effect of S5a-binding on hHR23a protein structure. S5a (196–307) contains the two ubiquitin-binding domains and was previously shown to bind hHR23a with equal affinity to full-length S5a . the union east rutherfordWebDec 15, 2009 · Decreased 19S-S5a protein levels and MGO-modified 20S-β2 proteasome subunit in GLO1-knockdown (GL01-KD) and STZ diabetic mice. Kidney lysate protein (40 … the union electric band

"WebS5a/Rpn10, a UIM-protein, as a universal substrate for ubiquitination The assay of the activity of ubiquitin (Ub) ligases (E3s) and screens for pharmacological agents that alter … " - S5a protein

S5a protein

Novel Regulation of the Helix-Loop-Helix Protein Id1 by S5a, a …

WebJan 1, 2005 · The S5a protein was immunoprecipitated from 3 mg of cell-lysate protein, and the immunoprecipitates were analysed for the presence of VCP, SAKS1, S4 and S5a by immunoblotting. Figures - uploaded ... WebApr 11, 2003 · Total Structure Weight: 9.52 kDa Atom Count: 668 Modelled Residue Count: 85 Deposited Residue Count: 85 Unique protein chains: 1 Display Files Download Files 1P1A NMR structure of ubiquitin-like domain of hHR23B PDB DOI: 10.2210/pdb1P1A/pdb Classification: DNA BINDING PROTEIN Organism (s): Homo sapiens Expression System: …

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WebS5a/Rpn10 is a major Ub-binding protein that binds preferentially to poly-Ub chains ( 13 ). It is found as a subunit of the 26S proteasome, but unlike other proteasome subunits, S5a exists predominantly as a free protein in the cytosol ( i.e. not incorporated into the proteasome) ( 14 , 15 ). WebApr 12, 2024 · Furthermore, we found that the gene expression levels of two membrane proteins, delta-like protein 1 (DLK1) and transmembrane protein 119 (Tmem119), were specific expressed in the Fibro_Pro-regen fibroblasts . DLK1 is a noncanonical ligand of Notch signaling, which mediates the vascular development and angiogenesis .

WebIdentification of the Ub-like sequence as the S5a-interacting domain of hHR23B raised the possibility that the S5a protein binds to any proteins which are conjugated with ubiquitin. To examine whether this is the case, we performed competition experiments using either free ubiquitin itself or the NH 2-terminal Ub-like domain of hHR23B. WebS5a/PSMD4 Antibody detects endogenous levels of total S5a/PSMD4 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly344 of human S5a/PSMD4 protein.

Web55 minutes ago · For fusion protein production, the DNA was transformed into BL21(DE3) competent cells for high protein expression and induced mid-log phase with 0.4 mM IPTG 16–20 h at 22 °C. WebJul 8, 2024 · Mass spectrometric analyses revealed a highly phosphorylated serine-rich (SR) motif at the C-terminal intrinsically disordered region of the P protein. The Ala-substitution mutant (P S5A) in the SR motif stimulated virus replication, whereas the phosphorylation-mimic mutant (P S5D) facilitated virus transcription.

WebDownload scientific diagram DNA and protein sequence of S5a. The sequence depicted encodes the entire S5a protein beginning with potential initiation Met codons at positions …

WebFeb 15, 2024 · MIST (protein-protein) - An integrated Molecular Interaction Database RpS5a Reactome - An open-source, open access, manually curated and peer-reviewed pathway … the union edinburghWebThe human protein shares 96% and 99% aa sequence identity with its mouse and rat orthologs, respectively. Structurally, it contains an N-terminal von Willebrand Factor type A domain and two C-terminal Ubiquitin-interacting motifs (UIM). It acts as a Ubiquitin-binding protein where it is most commonly referred to as S5a or in yeast as Rpn10. the union effect tucWebThe human protein shares 96% and 99% aa sequence identity with its mouse and rat orthologs, respectively. Structurally, it contains an N-terminal von Willebrand Factor type A domain and two C-terminal Ubiquitin-interacting motifs (UIM). It acts as a Ubiquitin-binding protein where it is most commonly referred to as S5a or in yeast as Rpn10. the union effectWebC5a protein is composed of alpha and beta polypeptide chains, which are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin, which has potent … the union electionWebSep 13, 2006 · The results suggest that the protein folding reaction is linked to topology only in the sense that the native-state topology determines the repertoire of accessible … the union edinburgh agencyWebS5a/Angiocidin, also known as Anti-secretory Factor (ASF), is classified under the gene PSMD4, but is often referred to by a different name depending on the context in which it is … the union electrical jobsWebProtein names Recommended name 26S proteasome non-ATPase regulatory subunit 4 Alternative names 26S proteasome regulatory subunit RPN10 26S proteasome regulatory subunit S5A Antisecretory factor 1 ( AF; ASF) Multiubiquitin chain-binding protein Gene names Name PSMD4 Synonyms MCB1 Organism names Organism Homo sapiens … the union esports