Mdpc6t cells

Author: eooc

August undefined, 2024

Web5 apr. 2013 · Furthermore, forced expression of Dmp1 in the Klf4 knock-down mDPC6T cell line significantly recovered its odontoblastic differentiation ability. Our data confirmed our … WebWe further examined the effect of DSP aa183-219 on cell migration. mDPC6T cells were stimulated with different concentrations of DSP aa183-219 or BSA as a control for 12 h. …

DSP aa183-219 promotes attachment, spreading, proliferation and ...

Web1 okt. 2013 · Based on further analysis of ChIP data, EMSA and dual luciferase activity assays, we confirmed that Klf4 was able to specifically bind to the Dmp1 promoter and … WebThe mDPC6T cells were transduced with pLVTHM-miR-145 and one of the lentivirus constructs described above, respectively. We observed that Klf4 or Osx without the 3′ … new hampshire 1909

LPS induced mitochondrial injury and mtDNA leakage into

Web1 mei 2024 · In this study we explored the anti-inflammatory function of FoxO3a-regulated autophagy in inflamed human dental pulp and lipopolysaccharide-treated mDPC6T cells. … Webthe pre-odontoblast cell line was obtained and named mDPC6T, which was maintained in the same condition as the primarilyculturedmDPC.The293FTcellline(humanembry-onic … WebB The mRNA level of CXCL10 and IFN-β in mDPC6T cells was assessed by qPCR with or without GSDMD knockdown in response to 24 h of mtDNA transfection. C The protein … new hampshire 1905

Autophagy flux was detected by mRFP-GFP-LC3 plasmid. Cells …

Phosphorylation of ATF2 promotes odontoblastic differentiation …

WebAbstract: Objective: To examine the function of XBP1s in inflammation-induced autophagy in odontoblasts. Methods: Immunohistochemistry was used to examine the expression of … WebCell culture and differentiation MDPC6T is a pre-odontoblast cell line established by our group (manuscripts submitted for publication). The 293FT cell line was purchased from … interviewer bias exampleWebMitochondrial function was impaired in inflamed mDPC6T cells, as indicated by the increased production of mtROS (Fig. 4b). In addition, in inflamed mDPC6T cells, intact … new hampshire 1928

"WebCytosolic DNA was assessed by immunofluorescent staining and real-time PCR in mDPC6T cells after LPS stimulation. Furthermore, mDPC6T cells were treated with ethidium … " - Mdpc6t cells

Mdpc6t cells

miR-3065-5p regulates mouse odontoblastic

WebLipopolysaccharide (LPS) is widely used for induction of inflammation in various human tissues, including dental pulp. The purpose of this study was to summarize current … WebWe conducted in vitro studies by treating mDPC6T cells with lipopolysaccharide for various lengths of time. Next, we measured the nuclear translocation of FoxO3a by …

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Web477 members in the InflammationStation community. A forum to identify, discuss, and promote research & treatment for the various types of … WebAbstract. Odontoblasts are terminally differentiated cells that play a vital role in dentinogenesis. The differentiation of odontoblasts is regulated by a variety of genetic …

WebCytosolic DNA was assessed by immunofluorescent staining and real-time PCR in mDPC6T cells after LPS stimulation. Furthermore, mDPC6T cells were treated with ethidium …

Web15 jun. 2024 · Mechanistically, RNA sequencing and m 6 A sequencing of Alkbh5-overexpressing mDPC6T cells revealed that Alkbh5 promoted odontoblast differentiation … WebQuantitative reverse transcription-polymerase chain reaction (qRT-PCR) and immunofluorescence revealed that the expression of SP1 was significantly upregulated during odontoblastic differentiation of mDPC6T cells, a dental papilla cell line.

Web9 dec. 2024 · mDPC6T cells were transfected with GSDMD-targeted small interfering RNA (GenePharma China, Shanghai). The cells were transfected twice using Lipofectamine …

Web1 jan. 2024 · Transfection was employed with Lipofectamine™ 2000 (Invitrogen). miR-3065-5p mimics was transfected into mDPC6T cells at a final concentration of 50 nM. 2.7. … new hampshire 1923Web24 aug. 2024 · 2.4. Cell Viability Test. mDPC6T cells were seeded in 96-well plates (1×10 4 cells/well) and cultured under different conditions, as indicated for each experiment. … new hampshire 1924WebQuantitative reverse transcription-polymerase chain reaction (qRT-PCR) and immunofluorescence revealed that the expression of SP1 was significantly upregulated … interviewer asked you about your job searchWebDSP aa183-219 promotes attachment, spreading, proliferation and migration of mouse dental papilla mesenchymal cells. (A) mDPC6T cells were seeded on DSP aa183-219 … new hampshire 1916Web9 dec. 2024 · We found that LPS stimulation of mDPC6T cells promoted BAX translocation from the cytosol to the mitochondrial membrane, leading to mtDNA release. Moreover, … interviewer bias sociologyWeb9 dec. 2024 · This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our … new hampshire 1922WebLipopolysaccharide (LPS) is widely used for induction of inflammation in various human tissues, including dental pulp. The purpose of this study was to summarize current medical literature focusing on (1) cell types used by researchers to simulate dental pulp inflammation, (2) LPS variants utilized in experimental settings and how these choices … interviewer ask tell me about yourself