Cytokinin dehydrogenase activity
WebMar 24, 2010 · Almost 80% of 52 regenerated lines of Golden Promise exhibited significantly decreased cytokinin oxidase/dehydrogenase (CKX) enzyme activity in bulked samples of their T 1 roots. There was a positive correlation between the enzyme activity and the plant productivity, expressed as the yield, the number of seeds per plant, and the 1000 grain … WebJul 1, 2002 · Spectrophotometric methods for determining the activity of cytokinin oxidase/cytokinin dehydrogenase (EC 1.5.99.12) were developed and optimized. A sensitive end-point method based on a combination of the electron acceptor 2,6-dichlorophenolindophenol and Schiff base formation of the reaction product with 4 …
Cytokinin dehydrogenase activity
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WebApr 9, 2024 · N 6-(2-Isopentenyl)adenine (2iP) and zeatin are natural N 6-substituted purine cytokinins that are used for in vitro culture protocols, mainly to promote axillary shoot proliferation and adventitious shoot induction.One of the mechanisms that regulates local cytokinin homeostasis in the in vitro plant is their degradation by cytokinin … Webcytokinin oxidase activity in the presence of copper-imidazole complexes was first observed by Chatfield and Armstrong [9]. This enhancement was not affected by anaerobic conditions, showing that the copper-imidazole complexes are substitutes for oxygen. Recently, the acti-vation of cytokinin oxidase by some phenolic compounds
WebThe size and activity of the shoot apical meristem is regulated by transcription factors and low molecular mass signals, including the plant hormone cytokinin. The cytokinin status of the meristem depends on different factors, including metabolic degradation of the hormone, which is catalyzed by cytokinin oxidase/dehydrogenase (CKX) enzymes. WebCytokinin group of phytohormones regulate root elongation and branching during post‐embryonic development. Cytokinin degrading enzymes cytokinin …
WebCytokinin measurements in ckx3 mutants confirmed that CKX3 activity negatively regulates root cytokinin levels. Particularly, tZ and DHZ type cytokinins in both inoculated and uninoculated roots were elevated in ckx3 mutants, suggesting that these are targets for degradation by the CKX3 cytokinin oxidase/dehydrogenase. The effect of CKX3 on the ... WebSep 28, 2007 · The biological activity of cytokinin nucleotides is still unclear, since they usually show no activity in bioassays but can bind to certain cytokinin receptors …
WebWendy A. Stirk mainly investigates Botany, Cytokinin, Axenic, Chlorophyceae and Zeatin. Wendy A. Stirk has included themes like Micropropagation and Tissue culture in her Botany study. ... antioxidant and antibacterial activity of Hypoxis hemerocallidea Fisch. & C.A. Mey (28 citations) ... Her work deals with themes such as Malate dehydrogenase ...
WebJan 5, 2024 · Cytokinin regulates the activity of reproductive meristems, flower organ size, ovule formation, and thus seed yield in Arabidopsis thaliana. Bartrina I, et al. Plant Cell, … hide your stand mixerWebMay 6, 2024 · Inhibitors of cytokinin oxidase/dehydrogenase (CKX) reduce the degradation of cytokinins in plants, and this effect can be exploited in agriculture … how far away is baltimore marylandWebThe larvicidal activity of natural inspired piperine-based dienehydrazides against Culex pipiens: ... Transcriptome profiling reveals cytokinin promoted callus regeneration in Brassica juncea: ... A quinone-dependent dehydrogenase and two NADPH-dependent aldo/keto reductases detoxify deoxynivalenol in wheat via epimerization in a Devosia strain: hide your thumbs when you pass a graveyardWebSep 16, 2015 · Cytokinin dehydrogenase activity was measured using a modified end-point method described earlier . The reaction mixture (total volume of 0.6 ml in 1.5 ml tube) consisted of 100 mM McIlvaine buffer, pH 5.0 or 7.5, electron acceptor, substrate (dissolved in dimethyl sulfoxide that reached the final concentration of 2.5% in the reaction mixture ... hide your stomachWebEven though, it has already been established since 1980s that the enzyme, cytokinin oxidase/dehydrogenase (CKO/CKX) deactivates cytokinins; the potential applications of manipulating these enzymes have mostly been speculated to have a high potential in the biotechnology industry and spreads to agriculture, horticulture and agroforestry ... how far away is beamishWebThe cytokinin zeatin is named after the genus of corn, Zea. Cytokinins ( CK) are a class of plant hormones that promote cell division, or cytokinesis, in plant roots and shoots. They … hide your sheep tourWebMar 29, 2024 · In addition, the degradation of cytokinin mainly depends on the activity of cytokinin oxidative dehydrogenase, which irreversibly inhibits cytokinin degradation (Yeh et al. 2015; Xiao et al. 2016). Morris et al. ( 1999 ) found that cytokinins are extensively degraded in the anthers and pollen of cytokinin oxidative dehydrogenase transgenic … hide your toaster