WebFeb 1, 2024 · To compare gene expression profiles of human and cynomolgus monkey PBMCs in response to PHA and LPS, the PBMCs (10 6 cells/mL) obtained from cynomolgus or human donors were placed in 6-well plates (10 6 cells/well), treated with PHA (2 or 10 µg/mL) or LPS (2 or 10 µg/mL), and incubated at 37 °C for 72 hours. WebMACS Cell Separation Cell separation reagents MicroBeads and Isolation Kits CD20 MicroBeads, non-human primate CD20 MicroBeads, non-human primate CD20 MicroBeads were developed for the positive selection or depletion of B cells from rhesus monkey PBMCs or single-cell suspensions from tissue. Product name Size Quantity List …
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WebApr 11, 2024 · For our experimental model, we identified eight SIV-infected cynomolgus monkeys that became natural controllers of viremia. Although plasma viral loads were undetectable, we could measure... WebMar 24, 2024 · In vivo, CYT-303 showed no toxicity or cytokine release in cynomolgus monkeys up to the highest dose (60 mg/kg), administered weekly by intravenous infusion for 28 days. ... Human PBMCs from multiple healthy donors were incubated with different concentrations of CYT-303 or anti-CD3 (Muromonab-CD3) or CD28 mAbs (TGN1412) … cycloplegics and mydriatics
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WebOct 22, 2024 · Cryopreserved splenocytes or peripheral blood mononuclear cells (PBMCs) from individual Balb/c mice (IQ Biosciences; Cupertino, CA), cynomolgus monkeys (IQ), or healthy consenting adult volunteer human donors (Genentech) were revived in RPMI 1640 media supplemented with 10% fetal bovine serum for a minimum of 2 h in a humidified … WebMay 19, 2016 · I have been getting quite a significant amount of RBCs in the PBMCs each time I isolate PBMCs from the blood of cynomolgus monkeys. This is how I do the … WebThe cryopreserved cynomolgus monkeys tissue cells, human and monkey PBMCs, and human cell lines were thawed on ice and centrifuged at 2000 xg for 6 min at 4° C. The supernatants were discarded and 1 mL RPMI 1640 medium (Sigma-Aldrich, Oakville, Canada) without FBS (fetal bovine serum) was added. cyclopithecus