4t1细胞培养注意事项
WebAug 4, 2024 · For 4T1 tumor-specific antibody detection, the blood of each group of BALB/c mice was collected from the orbital venous plexus at 4 weeks after tumor cell inoculation. Parental 4T1 cells were incubated with mouse serum (1:100 dilution) or PBS at 4°C for 30 min, and then stained with FITC-labeled goat antimouse antibody IgG. ...
4t1细胞培养注意事项
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WebThe 4T1 mammary carcinoma is a transplantable tumor cell line that is highly tumorigenic and invasive and, unlike most tumor models, can spontaneously metastasize from the primary tumor in the mammary gland to multiple distant sites including lymph nodes, blood, liver, lung, brain, and bone The 4T1 tumor has several characteristics that make it a … 4T1 is a breast cancer cell line derived from the mammary gland tissue of a mouse BALB/c strain. 4T1 cells are epithelial and are resistant to 6-thioguanine. In preclinical research, 4T1 cells have been used to study breast cancer metastasis as they can metastasize to the lung, liver, lymph nodes, brain and bone. The cells are known to be highly aggressive in live tissues.
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WebAug 12, 2015 · In this study, we investigated adverse effects of repeated administration of PD-1 and PD-L1 mAbs in the murine 4T1 mammary carcinoma model. We observed rapid and fatal hypersensitivity reactions in tumor bearing mice within 30-60 min after 4-5 administrations of PD-L1 or PD-1 mAb but not CTLA-4 antibody treatment. These events … http://www.immocell.com/xsxbx/339.html
WebDec 1, 2004 · 4T1 cells, a breast cancer cell line derived from a BALB/c mouse, were obtained from the American Type Culture Collection (Manassas, VA) and cultured in DMEM, 10% heat-inactivated fetal bovine serum, 1% glutamine, 0.1% fungizone, and 0.1% penicillin/streptomycin. 293T cells were cultured in the same medium.
WebApr 15, 2024 · 图7 放疗辅助atp反应的cpg释放水凝胶和免疫检查点阻断的原位4t1乳腺癌治疗. a）由atp反应的cpg释放水凝胶和免疫检查点阻断辅助放射治疗4t1乳腺癌原位治疗的示意图。 b）不同治疗方式下原发4t1肿瘤的生长曲线。 c）4t1小鼠在不同治疗后的存活率。 cotswold obituary4T1细胞是从410.4瘤株中未经诱变筛得的6-硫鸟嘌噙抗性细胞株。当4T1细胞注射到BALB/c小鼠中时，4T1细胞会自发产生高转移肿瘤，可转移到肺、肝、淋巴结和大脑，同时在注射部位形成始发灶，诱导转移时不需要摘除始发 … See more cotswold obscure glassWebNov 8, 2024 · 1）复苏细胞：将含有1mL细胞悬液的冻存管在37℃水浴中迅速摇晃解冻，加入5mL培养基混合均匀。 在1000RPM条件下离心5分钟，弃去上清液，补加4-6mL*培养基后吹匀。 然后将所有细胞悬液加入培养瓶中培养过夜（或将细胞悬液加入6cm皿中），培养过夜。 第二天换液并检查细胞密度。 2）细胞传代：如果细胞密度达80%-90%，即可进行传 … cotswold observerWebBecause 4T1 is resistant to 6-thioquanine, micro-metastatic cells (as few as 1) can be detected in many distant site organs with better accuracy that most tumor models. There is no need to count nodules or weight target organs. Handling information Unpacking and storage instructions Check all containers for leakage or breakage. cotswold ocean njWebC7218. 4T1 (小鼠乳腺癌细胞) 1支/瓶. —. 说明书. 1份. 保存条件：. 对于细胞培养瓶或离心管运输的活细胞，室温3-5天有效。. 对于干冰运输的冻存细胞，液氮保存，长期有效； … breathe watch appWeb1、加入消化液：小心吸出旧培养液，用PBS清洗（冲洗），加入适量消化液（胰蛋白酶液），注意消化液的量以盖住细胞最好，最佳消化温度是37℃。 2、显微镜下观察细胞：倒置显微镜下观察消化细胞，若胞质回缩，细胞之间不再连接成片，表明此时细胞消化适度。 3、吸弃消化液加入培养液：弃去胰蛋白酶液，注意更换吸管，加入新鲜的培养液。 胰蛋白酶 … breathe water 5ehttp://www.cailiaoniu.com/184993.html breathe wall art